Where does RNA editing occur?
RNA editing occurs in the nucleus, as well as in mitochondria and plastids, which are thought to have evolved from prokaryotic-like endosymbionts. Most of the RNA editing processes, however, appear to be evolutionarily recent acquisitions that arose independently.
What changes may occur due to RNA editing?
Location of RNA editing events in pre-mRNA transcripts and known functional implications. A: Editing within the open reading frame of a protein-coding transcript. RNA editing changes a codon, which in turn leads to a protein with amino acid substitution and altered functional properties.
Where does splicing happen?
For nuclear-encoded genes, splicing occurs in the nucleus either during or immediately after transcription. For those eukaryotic genes that contain introns, splicing is usually needed to create an mRNA molecule that can be translated into protein.
What does Apobec stand for?
APOBEC stands for apolipoprotein B mRNA-editing enzyme, catalytic polypeptide. As the name suggests, this class of enzyme was originally identified as an enzyme that edits mRNA species by deaminating cytosine to uracil, which in this case produces a stop codon and truncated protein (Anant & Davidson, 2001).
Where the central dogma occurs in eukaryotic and prokaryotic cells?
Prokaryotic transcription and translation occur simultaneously in the cytoplasm, and regulation occurs at the transcriptional level. Eukaryotic gene expression is regulated during transcription and RNA processing, which take place in the nucleus, and during protein translation, which takes place in the cytoplasm.
Where does Central Dogma occur in eukaryotes?
the nucleus
The Central Dogma in eukaryotic cells In eukaryotes (organisms with a nuclear membrane), DNA undergoes replication and transcription in the nucleus, and proteins are made in the cytoplasm. RNA must therefore travel across the nuclear membrane before it undergoes translation.
What does APOBEC stand for?
Where does APOBEC1 RNA editing occur in the brain?
At an experimental level mouse APOBEC1 is remarkable among 12 mammalian A1 enzymes in that it represents a source of somatic mutations in mouse genome, potentially fueling oncogenesis. APOBEC1-mediated RNA editing occurs within brain microglia cells and is key to maintaining their resting status.
What happens when ApoBEC-1 mRNA is deleted?
Deletion of apobec-1, by modulating expression of AU-rich RNA targets, provides an important mechanism for attenuating a dominant genetic restriction point in intestinal adenoma formation. apobec-1-mediated apoB mRNA editing is regulated by BAG-4
What is the role of APOBEC1 cytidine deaminase?
Results suggest that apo B mRNA editing protein (Apobec1 cytidine deaminase) plays a central role in controlling testicular germ cell tumors susceptibility in both a conventional and a transgenerational manner.
Where does transgenic rescue of apob-1 mRNA take place?
The transgenic rescue of intestinal apobec-1 expression restores C-to-U RNA editing of apoB mRNA in vivo, including the canonical site at position 6666 and also at approximately 20 other newly identified downstream sites present in WT mice.