What is the difference between agarose and sepharose beads?
Sepharose is also another polymer like agarose but slightly differs because it is more beaded in form. Pure agarose is powdered form while sepharose is more beaded in structure. 2. Agarose is a more generic term referring to a type of polysaccharide polymer while sepharose is a trademarked term by GE Healthcare.
What are Sepharose beads?
Sepharose Big Beads are ion exchangers designed for large-scale industrial applications. The media are based on 100–300 μM, cross-linked 6% agarose particles, substituted with quaternary ammonium (Q) or sulfopropyl (SP) groups.
How do you manufacture an agarose bead?
General method for making porous agarose beads. The agarose solid is dissolved in water heated to about 90° C. This is then added to a stirred vessel containing a hydrophobic solution (eg. Toluene or mineral oil) together with an emulsifier.
What are agarose beads used for?
Agarose beads are small spherical beads of agarose gel which are commonly used in gel filtration or molecular size exclusion chromatography and biomolecular purification and immobilization. These beads act as porous gel to filter mixtures of molecules based on their individual sizes.
What is Sepharose 4B?
Sepharose 4B is a well-proven agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
What is Protein A Sepharose?
Protein A Sepharose® beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose® beads. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. This product can be used for IgG purification and immunoprecipitation.
What is Blue Sepharose?
Blue Sepharose® 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors.
What is protein G Sepharose?
Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. Protein G Sepharose® beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
What is the chemistry of agarose beads?
Agarose is a high-molecular-weight polysaccharide extracted from the cell walls of certain marine red algae. Chemically, it is a copolymer of 1,3-linked β-d-galactose and 1,4-linked 3,6-anhydro-α-l-galactose.
What does protein G bind?
Streptococcal protein G is a cell wall protein with an extracellular part composed of two (or three) small domains that bind serum albumin (GA domains) and two (or three) immunoglobulin binding domains (B domains).
How big are the particles in agarose beads?
Agarose beads are available in different concentrations of agarose (2%, 4%, and 6%) that alter the separation range and bead size of the agarose beads. 2% agarose has a particle size ranging between 60-200µm while 4% and 6% have particles ranging between 45-165μm.
What kind of beads are used in SP Sepharose?
BioProcess resin supported for industrial applications and well-established in approved processes SP Sepharose Big Beads is composed of large crosslinked agarose beads (100-300 µm) modified with sulphonate (SP) strong cation exchange groups.
How are agarose beads used in affinity chromatography?
Agarose beads bind naturally not itself to proteins. They have to be attached to ligands such as NTA, glutathione, or antibodies, to enable purification of proteins via a specific interaction. For affinity chromatography the most used agarose beads are Nickel-NTA beads.
What kind of agarose is used for FPLC?
GE trademark for crosslinked agarose in different concentrations and sizes. Properties of Sepharose vary depending on agarose concentration and degree of cross-linking, and not all Sepharose matrices are suitable for applications such as FPLC. Offered by several companies.