What is Bulbosum technique?
bulbosum method, is a reliable technique that has been used for many years by researchers and breeders to generate thousands of DH lines of barley from which many cultivars have been derived and grown in several areas over the world (Devaux et al., 1996).
What is the difference between embryo culture and embryo rescue?
Plant Tissue Culture Embryo culture is the culture of isolated immature or mature embryos. To recover such a hybrid embryo, culturing of the embryo was followed. This culturing of embryo (hybrid) in in vitro conditions is known as embryo rescue and is widely used for crop improvement.
How does tissue culture produce Haploids?
The production of haploids through anther or pollen culture is called androgenesis and to-date, it has been reported in 135 species. The principle involved in the process is to halt the development of pollen cells into a gamete and induce it in a suitable environment to develop into a haploid plant.
What is embryo rescue Slideshare?
Embryo Rescue: • Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the development of plant embryos that might not survive to become viable plants. • Embryo rescue plays an important role in modern plant breeding and ornamental plant crop hybrids.
What is Bulbosum technique of haploid production?
Since 1970 it has been used in the Hordeum bulbosum Method (or Technique) to produce doubled haploid (DH) wheat and barley plants by crossing it with T. aestivum or H. vulgare, followed by the elimination of the H. bulbosum chromosomes from the offspring.
What is double haploid technology?
A doubled haploid (DH) is a genotype formed when haploid cells undergo chromosome doubling. Conventional inbreeding procedures take six generations to achieve approximately complete homozygosity, whereas doubled haploidy achieves it in one generation.
What is embryo rescue technique?
An embryo-rescue procedure involves three main steps: (1) ovules are cultured in vitro (embryo formation), (2) embryos are removed from the ovules and cultured (embryo germination and plantlet development), and (3) plantlet roots are elongated, acclimated, and transplanted to soil (Pan 2005; Tang 2010; Wang et al.
What techniques use embryo culture?
Embryo culture is a component of in vitro fertilisation where in resultant embryos are allowed to grow for some time in an artificial medium .
What techniques eradicate viruses?
In vitro thermotherapy-based methods, including combining thermotherapy with shoot tip culture, chemotherapy, micrografting or shoot tip cryotherapy, have been successfully established for efficient eradication of various viruses from almost all of the most economically important crops.
How are haploids developed?
Haploid cells are produced from pollen or egg cells or from other cells of the gametophyte, then by induced or spontaneous chromosome doubling, a doubled haploid cell is produced, which can be grown into a doubled haploid plant.
What is embryo rescue technique mention its use?
Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the development of plant embryos that might not survive to become viable plants. This technique nurtures the immature or weak embryo, thus allowing it the chance to survive.
Which of the following is known as Monoploidy?
In a diploid state the haploid number is doubled, thus, this condition is also known as 2n. A monoploid would only have one set of the chromosome and therefore is represented by 1n. For instance, certain male wasps, ants, and bees are monoploid and have developed from unfertilized eggs.
How is the Hordeum bulbosum ( L ) method used?
The Hordeum bulbosum (L.) method. This chapter describes a protocol for the interspecific hybridization of H. vulgare with H. bulbosum, also called the H. bulbosum method that has been used at Florimond Desprez for twenty years to generate DH lines of barley. H. bulbosum. is a perennial outcrossing species found in the Mediterranean region.
Why is the bulbosum method used in barley breeding?
Bulbosum method consists in the interspecific hybridization of cultivated barley (Hordeum vulgare L.) with Hordeum bulbosum L. (Kasha & Kao, 1970). Scientists and breeders rapidly adopted the bulbosum method because DH (doubled haploids) provide several advantages over standard selection and breeding methods (Devaux, 2003).
Can You hybridize h.vulgare with h.bulbosum?
This chapter describes a protocol for the interspecific hybridization of H. vulgare with H. bulbosum, also called the H. bulbosum method that has been used at Florimond Desprez for twenty years to generate DH lines of barley. H. bulbosum. is a perennial outcrossing species found in the Mediterranean region.
Where can I find Hordeum bulbosum in the world?
H. bulbosum. is a perennial outcrossing species found in the Mediterranean region. Although there are two cytotypes, diploid (2n = 2x =14) and tetraploid (2n = 4x = 28), only the diploid form has been used to generate haploid plants of barley.