What color is BODIPY?
Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.
What is BODIPY staining?
With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.
Is BODIPY polar or nonpolar?
BODIPY Fluorophores BODIPY fluorophore derivatives offer an unusual combination of nonpolar structure and long-wavelength absorption and fluorescence.
How do you dissolve BODIPY?
Dissolve 1.3 mg BODIPY in 1 ml DMSO and can be stored at −20 °C. 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.
Is BODIPY a fluorescent?
Is BODIPY aromatic?
3. BODIPYs fused to aromatic heterocycles. So far, instead of aromatic hydrocarbons, many aromatic ring-fused BODIPY dyes have been developed by introducing 5-membered aromatic heterocycles, such as furan, thiophene and pyrrole derivatives, at the b bond, as summarized in Fig.
Can bodipy be fixed?
We routinely add BODIPY 493/503 from a 1 mg/ml stock to fixed or living cells at a final concentration of 1 μg–10 μg/ml; followed by at least a 1-h incubation at 37°C. The final concentration of BODIPY 493/503 depends on fat droplet size, the larger the fat droplets, the less stain is needed.
What is Nile red dye?
Nile red is a hydrophobic and metachromatic dye with poor solubility and fluorescence in water, with colour emission varying from deep red to strong yellow gold in hydrophobic environments. Depending on excitation and emission wavelength, the dye has been used to stain different hydrophobic molecules.
Is BODIPY cell permeable?
To test our hypothesis, we chose boron-dipyrromethene (BODIPY) as the model fluorescent dye in our study. However, BODIPY indeed suffer problems of low-water solubility and poor membrane permeability27,28.
What is BODIPY used for?
BODIPY dyes have been used extensively in recognition and imaging applications owing for their improved stability toward photobleaching as well as high quantum yield (Φ) and high molar absorption coefficient (ɛ) values.
What is Nile red staining?
Can BODIPY be fixed?
Why are BODIPY dyes used in fluorescent probes?
BODIPY dyes are also more useful than most other long-wavelength dyes, including fluoresceins and carbocyanines, for assays that measure fluorescence polarization ( Fluorescence Polarization (FP)—Note 1.4 ), and they have large cross-sections for excitation by multiphoton excitation sources ( Fluorescent Probes for Two-Photon Microscopy—Note 1.5 ).
How is BODIPY FL dye similar to FITC?
Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.
What are the emission maxima of BODIPY dyes?
However, when substituents that yield additional conjugation are added to the parent molecule, both the absorption and emission spectra of the resulting derivatives can shift to significantly longer wavelengths, with emission maxima of greater than 750 nm now possible with some BODIPY derivatives.
When does the BODIPY 581 / 591 fluorophore turn green?
The red fluorescence of the BODIPY 581/591 fluorophore shifts to green fluorescence upon peroxidation, a unique feature that has been exploited for ratiometric measurements of lipid oxidation in live cells ( Generating and Detecting Reactive Oxygen Species—Section 18.2 ).