How much does DMEM cost?
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| Product | Product Name | Price |
|---|---|---|
| SH30022.LS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 1000 mL | 192.19 USD |
| SH30022.01 | DMEM/High glucose with L-glutamine; without sodium pyruvate – 500 mL | 22.25 USD |
| SH30022.FS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 500 mL | 120.07 USD |
What is DMEM culture media?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
How much glucose is high in DMEM?
This product – DMEM High Glucose – has an increased glucose content of 4.5 g/L and is supplemented with L-glutamine. Dulbecco’s Modified Eagle Medium is based on Eagle’s Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins.
What is DMEM media used for?
What is in DMEM F12 media?
DMEM/F12 Medium is a 1:1 mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium, commonly used in growing a variety of mammalian cultures. DMEM/F12 is rich and complex, containing all 21 amino acids, 10 vitamins, glucose, zinc, and iron among other components.
What is DMEM 10?
Dulbecco’s modified Eagle’s medium (DMEM; GIBCO/Invitrogen) 10% normal calf serum. 1% penicillin/streptomycin (Cellgro) 1% L-glutamine (Cellgro) Filter-sterilize through a 0.22-μm filter.
What is F12 in DMEM F12?
Ahvaz Jundishapur University of Medical Sciences. DMEM/F-12 is a 1:1 mixture of Dulbecco’s modified Eagle medium and Ham’s F-12 medium.
What makes DMEM unique from other essential media?
The complete formulation is available. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
What do you need to know about DMEM supplementation?
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH. One moment while we fetch your results.
What kind of cells can be grown in DMEM?
Gibco Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
How much glucose is in DMEM high glucose?
Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”. This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate.