Can you Gram stain Chlamydia trachomatis?
Cell Structure and Metabolism Both Chlamydia trachomatis and Chlamydia pneumoniae are Gram-negative (or at least are classified as such, they are difficult to stain, but are more closely related to Gram-negative bacteria), aerobic, intracellular pathogens.
What stain is used for chlamydia?
While its sensitivity for detection of chlamydial inclusions in McCoy cells is equal to that of Giemsa staining, toluidine blue staining is easier and faster to perform. staining combined with dark field microscopy for detecting C trachomatis inclusions.
How is Chlamydia trachomatis detected?
A sample of your urine is analyzed in the laboratory for presence of this infection. A swab. For women, your doctor takes a swab of the discharge from your cervix for culture or antigen testing for chlamydia. This can be done during a routine Pap test.
Which test is preferred for detecting Chlamydia trachomatis?
There are several types of tests that can be used to detect chlamydia, including molecular testing, also called Nucleic Acid Amplification Test (NAAT), and cell culture. NAAT is the preferred method for detecting a chlamydia infection.
Why is chlamydia difficult to Gram stain?
Like other gram-negative bacteria, chlamydiae have an outer membrane that contains lipopolysaccharide and membrane proteins but their outer membrane contains no detectable peptidoglycan, despite the presence of genes encoding proteins for its synthesis.
Is Giemsa stain used for chlamydia?
The examination of Giemsa-stained smears for chlamydial inclusions is a useful technique for the diagnosis of trachoma or inclusion conjunctivitis by laboratories that do not have the specialized facilities for the identification of these chlamydial infections by the technically more complex procedures of …
How do you stain chlamydia?
Staining with iodine can distinguish between inclusion bodies of C trachomatis and C psittaci, as only the former contain glycogen. Each chlamydial agent can also be identified by using specific immunofluorescent antibodies prepared against either C trachomatis or C psittaci.
Is chlamydia a virus or bacteria?
Chlamydia (kluh-MID-e-uh) trachomatis (truh-KOH-muh-tis) is a common sexually transmitted infection (STI) caused by bacteria. You might not know you have chlamydia because many people don’t have signs or symptoms, such as genital pain and discharge from the vagina or penis.