What signaling pathway is beta catenin involved in?
Canonical pathway The canonical Wnt pathway (or Wnt/β-catenin pathway) is the Wnt pathway that causes an accumulation of β-catenin in the cytoplasm and its eventual translocation into the nucleus to act as a transcriptional coactivator of transcription factors that belong to the TCF/LEF family.
What is the role of beta-catenin?
β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene.
Where is B catenin found?
This protein is present in many types of cells and tissues, where it is primarily found at junctions that connect neighboring cells (adherens junctions). Beta-catenin plays an important role in sticking cells together (cell adhesion) and in communication between cells.
Is beta catenin a maternal?
The dorsal-specific gene Siamois was used as control for proper dorsal–ventral dissection. Consistent with Siamois being a direct target of maternal β-catenin, its expression was largely unaffected by CHX.
What genes does beta catenin regulate?
β-catenin target genes have been implicated in regulating different cellular processes including proliferation (e.g., MYC, CCND1, PPARD), stem cell fate (ASCL2), survival (ABCB1, BIRC5), differentiation (ID2, ITF2, ENC1), migration (MMP7, MMP14), and angiogenesis (VEGF) [4–18].
Which is the target gene for β-catenin?
DNA Libraries were prepared using SimpleChIP ® ChIP-seq DNA Library Prep Kit for Illumina ® #56795. The figure shows binding across chromosome 17 (upper), including AXIN2 (lower), a known target gene of β-Catenin (see additional figure containing ChIP-qPCR data).
What happens to β catenin in HeLa cells?
The change in β-Catenin molecular weight in the mutated HeLa cells is consistent with an in-frame deletion. Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP ® Rabbit mAb. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP ® Rabbit mAb.
Is there an antibody that detects total β catenin?
β-Catenin Antibody detects endogenous levels total of β-catenin.
Which is a downstream effector of the Wnt signaling pathway?
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β (4-6).