Which enzyme is useful in synthesis of dihydrofolic acid?
The sulfonamides competitively inhibit dihydropteroate synthetase, a vital enzyme that facilitates p-aminobenzoic acid as a substrate for the synthesis of dihydrofolic acid.
What is dihydrofolic acid reductase?
Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry.
What is Dihydrofolic acid used for?
Dihydrofolic acid is a folic acid derivative acted upon by dihydrofolate reductase to produce tetrahydrofolic acid. It interacts with bacteria during cell division and is targeted by various drugs to prevent nucleic acid synthesis. It has a role as an Escherichia coli metabolite and a mouse metabolite.
What is the biochemical function of Thfa?
THFA plays a key role in the transfer of 1-carbon units (such as methyl, methylene, and formyl groups) to the essential substrates involved in the synthesis of DNA, RNA, and proteins. More specifically, THFA is involved with the enzymatic reactions necessary to synthesis of purine, thymidine, and amino acid.
What is the function of Tetrahydrofolate?
Tetrahydrofolate is the main active metabolite of dietary folate. It is vital as a coenzyme in reactions involving transfers of single carbon groups. Tetrahydrofolate has a role in nucleic and amino acid synthesis.
How is folic acid converted to Tetrahydrofolate?
In the cells, folic acid is reduced to THF, a biologically active form, in a two-step process that requires two molecules of NADPH and the enzyme dihydrofolate reductase (DHFR).
What is the primary inhibitor of DHFR?
Methotrexate. (MTX) is a folate analogue and works by inhibiting dihydrofolate reductase (DHFR) resulting in the inhibition of rapidly dividing cells.
What is Thfa?
Tetrahydrofurfuryl alcohol (THFA) is an organic compound used as a solvent in many applications and products intended for both professionals (a chemical intermediate in the chemical and pharmaceutical industries, a solvent in pesticides) and consumers.
How does dihydrofolic acid interact with folic acid?
Dihydrofolic acid is a folic acid derivative acted upon by dihydrofolate reductase to produce tetrahydrofolic acid. It interacts with bacteria during cell division and is targeted by various drugs to prevent nucleic acid synthesis. It has a role as an Escherichia coli metabolite and a mouse metabolite.
What kind of acid is 7, 8-dihydrofolic acid?
Dihydrofolic acid, also known as 7, 8-dihydrofolate or H2PTEGLU, belongs to the class of organic compounds known as glutamic acid and derivatives.
Where is dihydrofolic acid found in the body?
Dihydrofolic acid is considered to be a practically insoluble (in water) and relatively neutral molecule. Dihydrofolic acid has been found in human liver tissue, and has also been primarily detected in blood. Within the cell, dihydrofolic acid is primarily located in the mitochondria and lysosome.
What is the role of dihydrofolic acid in cell division?
It interacts with bacteria during cell division and is targeted by various drugs to prevent nucleic acid synthesis. It has a role as an Escherichia coli metabolite and a mouse metabolite. It is a conjugate acid of a dihydrofolate (2-).