What type of media is Thayer-Martin?
Thayer-Martin agar is a selective medium used for the isolation of gonococci (Neisseria gonorrheae) from specimens containing a mixed flora of bacteria and/or fungi e.g. urogenital specimen. Modified Thayer-Martin (MTM) agar is a GC agar base containing vancomycin, colistin, nystatin, and trimethoprim lactate (VCNT).
What is Thayer-Martin media used for?
Thayer-Martin Selective Agar is used for the isolation of pathogenic Neisseria from specimens containing mixed flora of bacteria and fungi. Carpenter and Morton described an improved medium for the isolation of the gonococcus in 24 h.
Does chlamydia grow on Thayer-Martin?
Clinical implications. A negative culture on Thayer-Martin in a patient exhibiting symptoms of pelvic inflammatory disease most likely indicates an infection with Chlamydia trachomatis.
What are the components of Thayer-Martin agar?
Trimethoprim lactate refrains Proteus swarming. The resulting medium is called Modified Thayer Martin….Composition.
| Ingredients | |
|---|---|
| Dipotassium Phosphate | 4.0g |
| Monopotassium Phosphate | 1.0g |
| Sodium Chloride | 5.0g |
| Agar | 12.0g |
What is modified Thayer Martin Agar?
Thayer Martin Agar (Modified) contains nystatin which has proven to be effective in the suppression of Candida albicans. Trimethoprim lactate has been added to suppress the swarming of Proteus species. Dextrose is added to enhance the growth of gonococci.
What is Martin Lewis Agar?
Martin Lewis Agar is a selective and enriched medium for the isolation and cultivation of Neisseria species from mixed flora. Hemoglobin, Bio-X, and dextrose incorporated in the culture media provide enough nutrients agents to permit luxuriant growth of fastidious microorganisms.
What antibiotics are in Thayer Martin Agar?
Our Thayer-Martin supplement contains four antibiotics: vancomycin which inhibits gram-positive organisms, colistin which inhibits gram-negative bacteria, nystatin which inhibits yeasts and molds, and trimethoprim which is primarily added to inhibit swarming Proteus species. 1. Allow medium to reach room temperature.
What culture media is used for chlamydia?
For successful culture of chlamydiae, swabs, scrapings and small tissue samples should be forwarded to the laboratory in a special chlamydial transport medium such as 2SP (0.2 M sucrose-phosphate transport medium containing 10 µg of gentamicin/mL, 25 µg of vancomycin/mL and 25 U of nystatin/mL).
Will chlamydia grow on blood agar?
Chlamydia species are obligate intracellular bacteria that require growth inside mammalian cells for propagation and survival. As a result, Chlamydia cannot be grown on conventional bacteriological medium.
Why do Thayer-Martin and Martin Lewis media have antibiotics added?
Why do Thayer-Martin and Martin-Lewis media have antibiotics added? To prevent overgrowth of the normal florae and to enhance the recovery of the pathogenic species, a selective medium containing inhibitors (antibiotics) for gram-negative and gram-positive organism and yeast is added.
What is the anti fungal agent used in Martin Lewis Media?
The selective medium comprises of the antimicrobial agent’s vancomycin, colistin, anisomycin (V-C-A Inhibitor) and trimethoprim, to suppress the normal flora. Vancomycin is active primarily against gram-positive bacteria.