What is the role of eIF4E?
eIF4E is the cap-binding protein which, in synergy with proteins such as the helicase eIF4A and the scaffolding protein eIF4G, binds to mRNA, allowing the recruitment of ribosomes and translation initiation.
What is the major function of the eukaryotic initiation factor 4E eIF4E during cellular translation?
Eukaryotic initiation factor 4E (eIF4E), a fundamental effector and rate limiting element of protein synthesis, binds the 7-methylguanosine cap at the 5′ end of eukaryotic messenger RNA (mRNA) specifically as a constituent of eIF4F translation initiation complex thus facilitating the recruitment of mRNA to the …
What phosphorylates eIF4E?
eIF4E is phosphorylated by the MNK1/2 serine/threonine kinases, which are activated in response to mitogenic and stress signaling downstream of ERK1/2 and p38 MAP kinase, respectively (9, 10). eIF4E phosphorylation at serine 209 by MNK1/2 promotes its transformation activity (11, 12).
Which eukaryotic initiation factor is known as cap-binding protein?
initiation factor-4E
The mRNA cap-binding protein, eukaryotic initiation factor-4E (eIF-4E), recognizes the mRNA cap structure and regulates translation. Insulin activation induces the phosphorylation of serine in eIF-4E, which in turn increases the affinity of eIF-4E for the mRNA cap structure and increases translation.
What is the role of elf4e in Polysomal translation?
Eukaryotic initiation factor eIF4E plays a pivotal role in translation initiation. As a component of the ternary eIF4F complex, eIF4E interacts with the mRNA cap structure to facilitate recruitment of the 40S ribosomal subunit onto mRNA.
What is the importance of eIF4E factor?
eIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Almost all cellular mRNA require eIF4E in order to be translated into protein.
What are the initiation factors involved in eukaryotic translation?
It requires at least 9 eukaryotic initiation factors (eIFs; Table 1) and comprises two steps: first, formation of 48S initiation complexes with established codon-anticodon base-pairing in the P-site of the 40S ribosomal subunits, and second, joining of 48S complexes with 60S subunits.
What is eif4?
What kind of antibody detects total eIF4E protein?
eIF4E Antibody detects endogenous levels of total eIF4E protein. The antibody does not cross-react with other proteins.
How does eIF4E affect cellular homeostasis in vivo?
However, it is believed that particular features of select mRNAs such as 5′ untranslated region (UTR) length, structure or sequence render such transcripts sensitive to eIF4E dose. We previously showed that, surprisingly, reduction of the dose of eIF4E in vivo does not affect global protein synthesis and cellular homeostasis 12.
What is the role of eIF4E in lipid metabolism?
Here,we pinpoint a surprising function for gene regulation at the step of translational control in obesity-associated lipid processing, breakdown, storage and LD growth. eIF4E is the major cap-binding protein that binds to the 5′ cap of all eukaryotic messenger RNAs to promote protein synthesis.
What happens to eIF4E + / − mice on high fat diet?
Initially we challenged eIF4E+/− mice with a high-fat diet (HFD, 60% of total calories from fat), which leads to obesity and metabolic syndrome in WT mice 3, 13. Unexpectedly, eIF4E+/− mice gained significantly less weight than their WT littermates after only 4 weeks on HFD (Fig. 1b ).