## What is the importance of tailing factor in HPLC?

All Answers (2) Symmetrical peaks with tailing factor of 1( this is the ideal tailing factor of course) results in accurate intergration of the peak area and also peak hight specailly in quantitative analysis.

### What is a good tailing factor?

Acceptable Tailing A new column is considered acceptable if the As value is 0.9 – 1.2 (0.9 indicates slight fronting). In practical terms, an As value below 1.5 is usually OK to work with, and up to As = 2.0 may be acceptable depending on the separation and resolution of the peaks.

#### How do you calculate tailing factor in HPLC?

The tailing factor is determined by drawing a perpendicular line from the peak centre to the baseline of the peak. Then the peak width and the front half-width are measured for the peak at 5% of the height of the peak. The tailing factor is simply the entire peak width divided by twice the front half-width.

**What is fronting and tailing?**

The chromatographic peak in (a) is an example of tailing, which occurs when some sites on the stationary phase retain the solute more strongly than other sites. The peak in (b) is an example of fronting, which most often is the result of overloading the column with sample.

**What is peak tailing in HPLC?**

Peak tailing is the most common chromatographic peak shape distortion. Peak tailing occurs when the peak asymmetry factor (As) is greater than 1.2 — although peaks with As greater than 1.5 are acceptable for many assays.

## Why peak tailing occurs in HPLC?

The primary cause of peak tailing is the occurrence of more than one mechanism of analyte retention. Secondary analyte interactions, with ionised silanols on the silica surface, give rise to peak tailing. These interactions need to be minimised to achieve acceptable peak shapes.

### How is USP tailing calculated?

The Tailing Factor is defined by the USP as the distance from the front edge of the peak to the back edge, divided by the distance from the front edge to the centerline, with all distances measured at 5% of the maximum peak height.

#### What is difference between asymmetry and tailing factor?

The USP tailing factor is widely accepted and differs from other asymmetry calculations in that it uses the peak width at 5% of peak height divided by 2x the front width at 5%. Asymmetry is commonly calculated as the ratio of back to front width at a specified % of peak height, normally at 10%.

**How do you calculate peak tailing?**

This is determined using the following equation: As = B / A; where B = peak width after the peak centre at 10% peak height; and A = peak width at baseline before the peak centre, The primary cause of peak tailing is the occurrence of more than one mechanism of analyte retention.

**What is peak tailing?**

## How do you calculate tailing factor?

It is calculated using the following equation: Tf = (a+b)/2a where a is the distance from the leading edge of the peak to the peak midpoint (perpendicular from the peak highest point) measured at 5% of peak height and b is the distance from the peak midpoint (perpendicular from the peak highest point) to the trailing …

### What to do about peak tailing in HPLC?

Changing the mobile phase organic modifier (ie: Acetonitrile vs Methanol) may improve peak shape due to secondary interactions. If you have excessively long tubing lengths between the HPLC column and detector, consider replacing them with shorter tubing lengths.

#### What is the asymmetry factor for peak tailing?

Peak tailing occurs when the peak asymmetry factor (As) is greater than 1.2 — although peaks with As greater than 1.5 are acceptable for many assays. This is determined using the following equation: As = B / A; where B = peak width after the peak centre at 10% peak height; and A = peak width at baseline before the peak centre,

**Why does peak tailing occur in secondary analyte interactions?**

Secondary analyte interactions, with ionised silanols on the silica surface, give rise to peak tailing. These interactions need to be minimised to achieve acceptable peak shapes. If you need assistance choosing the right column, then our HPLC column selection guide will be of help.

**What does the peak width of a HPLC mean?**

Peak width (w) The peak width covers the period from the beginning of the signal slope until reaching the baseline after repeated drop in the detector signal.

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