BookRiff

If you don’t like to read, you haven’t found the right book

What is Rox reference dye?

What is ROX dye? ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product.

What Colour is Rox?

ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.

What is reference dye in PCR?

ROX passive reference dye is an inert additive that provides a constant fluorescent signal for sample normalization throughout the real-time qPCR assay. Related Topics: qPCR Instrumentation, qPCR/Real-Time PCR Reagents, and qPCR Assay Design and Optimization.

What is a reference dye?

Description. ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.

What Rox means?

(slang, informal) Alternative spelling of rocks. ( in sense of excelling, being great)

What is ROX dye used for?

ROX is a fluorophore that can be used as a reference dye to normalize fluorescence across all of your samples (experimental and controls) for your instrument providing you a baseline. Essentially you just include a small, constant amount in all of your samples as a control.

At what close proximity the reporter dye and quencher are arranged in TaqMan probe?

Hydrolysis (TaqMan) Probes The hydrolysis probe is labeled with a fluorescent reporter at the 5′ end and a quencher at the 3′ end.

Is ROX a real word?

No, rox is not in the scrabble dictionary.

What does Rox mean in algebra?

Run Of eXperiments. Academic & Science » Electronics.

What is the purpose of Rox reference dye?

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.

Can You normalize PCR data with Rox dye?

Normalization of real-time PCR data using ROX or other passive reference dye also reduces, to a small extent, the effects on data of pipetting or other volume errors.

Can a Rox dye be used on a prism?

This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye: For Research Use Only. Not for use in diagnostic procedures. Store in freezer (-5 to -30°C).

What kind of dye is used in qPCR?

What is ROX dye? ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product.