What is Alcian blue PAS stain?
Alcian Blue/PAS Stain Kit is intended to identify acidic and neutral mucins in tissue sections on the Artisan Link and Artisan Link Pro Staining Systems. Alcian Blue pH 2.5 stains the acid mucin blue while the Schiff’s reagent stains the neutral mucins pink to red.
What is the staining procedure of PAS stain?
Procedure for Periodic Acid-Schiff (PAS) Staining Rinsing: In distilled water. Aldehydration: Place the stain in Schiff reagent for 15 minutes, which turns light pink. Washing: Using lukewarm tap water, wash the stain for 5 minutes, turning it dark pink. Counterstaining: Add Mayer’s Hematoxylin for 1 minute.
What is Alcian blue PAS used for?
PAS-Alcian Blue technique is used to differentiate between acidic mucins as sialomucins, sulfomucin and neutral epithelial mucin. After staining, acidic mucins will be coloured blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour.
Why would you perform a combined Alcian blue PAS stain?
The combination of the Alcian blue and the PAS techniques can be used as a means of distinguishing neutral mucins from acid mucins. The subsequent application of the PAS technique will stain the neutral mucins bright magenta.
How do you make Alcian blue solution?
Alcian blue solution, pH 2.5: Dissolve 1 g of Alcian blue (8GX) in 100 ml of 3% acetic acid solution….
- Deparaffinize and hydrate to distilled water.
- Stain in Alcian blue solution, pH 1.0 for 30 min.
- Blot sections dry with filter paper without rinsing in water.
What is the PAS stain used for?
PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.
Does Alcian blue stain goblet cells?
Definition of Barrett’s Esophagus An Alcian blue stain at pH 2.5 stains the acidic mucin present in the goblet cells. These cells commonly have a blush of eosinophilia, due to neutral mucin, on H&E stained sections, in contrast to the blush of basophilia, imparted by the acidic mucin in goblet cells.
What is alcian blue technique?
Standard alcian blue technique. Alcian blue is a large conjugated dye molecule that initially was used for the dyeing of textile fibers. This accounts for the staining of the proteoglycan/hyaluronic acid components of connective tissue and cartilage with alcian blue at a pH of 2.5.
What is PAS stain used for?
The PAS is most commonly used to demonstrate the thickness of glomerular basement membrane when renal disease is being assessed. The PAS staining procedure is also used to demonstrate hyphae and yeast-forms of fungi in tissue samples due to the high carbohydrate content of the organism’s cell walls.
What is the principle of Alcian blue stain?
PRINCIPLE: Alcian blue is a group of polyvalent basic dyes that are water soluble. The blue color is due to the presence of copper in the molecule. The 3% acetic acid solution (pH2. 5), Alcian blue stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
Why is Alcian blue used for PAS staining?
The rationale of the technique is that by first staining all the acidic mucins with Alcian blue, those remaining acidic mucins which are also PAS positive will be chemically blocked and will not react further during the technique. Those neutral mucins which are solely PAS positive will subsequently be demonstrated in a contrasting manner.
Can a neutral mucin be stained with alcian blue?
The Alcian blue at a pH of 2.5 will stain all acid mucins deep blue but will not color the neutral mucins. The subsequent application of the PAS technique will stain the neutral mucins bright magenta. Tissues or cells that contain both neutral and acidic mucins may demonstrate a dark blue or purple coloration (Figure 4).
What makes Alcian blue a water soluble dye?
PRINCIPLE:Alcian blue is a group of polyvalent basic dyes that are water soluble. The blue color is due to the presence of copper in the molecule. The 3% acetic acid solution (pH2.5), Alcian blue stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
When to use colloidal iron with alcian blue?
However, like Alcian blue the colloidal iron technique may be used in combination with the periodic acid also known as the Schiff technique. The PAS technique is perhaps the most versatile and widely used of the techniques for the demonstration of glycoproteins, carbohydrates and mucins.