What can GFP detect?
Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.
Can GFP detect antibodies?
GFP is a 27 kilodalton protein that can be detected in cells both in vivo and in vitro for studies in gene expression. Consult the supplier page to verify the identity of the desired antibody target and learn more detailed product information, such as species reactivity, antibody features, and validated applications.
Does GFP antibody recognize RFP?
A: The anti-GFP antibody was made using the N-terminal of GFP as an antigen and will not react with RFP proteins dsRED or mCherry, since their sequences are so different.
Can GFP antibody detect EGFP?
GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
How is GFP fluorescence detected?
Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is an effective and sensitive technique to quantitatively analyze fluorescent intensity, while fluorescent microscopy can visualize the subcellular location and expression of GFP.
Does GFP antibody recognize BFP?
Our GFP antibody does cross-react with the many fluorescent proteins that are derived from the jellyfish Aequorea victoria. These are all proteins that differ from the original GFP by just a few point mutations (EGFP, YFP, mVenus, CFP, BFP etc.).
What do anti-GFP antibodies do?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Does RFP antibody recognize mCherry?
The anti-RFP antibody 5F8 recognizes common RFP variants including mCherry, mPlum, mRFP, mRFPruby. “The 5F8 antibody is great.
What is GFP and RFP?
Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) are ~29 kDa fluorescent proteins used as versatile markers for visualization of protein localization, monitoring physiological processes, and for detecting transgenic or transient protein expression.
What does YFP mean?
Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria.