What is a pGEX plasmid?

Plasmid: pGEX-1 Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.

What is pGEX 2T plasmid?

Bacterial vector for expressing GST fusion proteins with a thrombin site.

What is pGEX?

pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.

What are expression vectors PPT?

Expression Vectors: Vectors that can yield the protein products of the cloned genes. Two elements that are required for active gene expression: a strong promoter and a ribosome binding site near an initiating ATG codon.

What is a pET vector?

pET Bacterial Recombinant Protein Vector. Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.

What is expression vector with example?

An example of expression vector is the plasmid used to produce insulin important for treating diseases such as diabetes. Another example is the expression vector that introduces specific genes necessary for the synthesis of beta-carotene into the cells of rice plants, giving rise to a new variety called golden rice.

What is difference between plasmid and vector?

Main Difference – Plasmid vs Vector The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell. Plasmids can also be used as vectors.

What is plasmid and its type?

A plasmid is a small, circular piece of DNA that is different than the chromosomal DNA, which is all the genetic material found in an organism’s chromosomes. It replicates independently of chromosomal DNA. Plasmids are mainly found in bacteria, but they can also be found in archaea and multicellular organisms.

What is plasmid and its types Slideshare?

Autonomously replicating circular fragment present in DNA is called plasmids. The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952. An episome is a plasmid capable of inserting DNA into the host chromosome.

What makes a plasmid an expression vector?

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell’s mechanism for protein synthesis to produce the protein encoded by the gene.

What are the three vectors of the pGEX vector?

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site (Figure 1.1). pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries.

How are GST-tagged proteins constructed in pGEX vectors?

GST-tagged proteins are constructed by inserting a gene or gene fragment into the MCS of one of the 13 pGEX vectors. Expression is under the control of the tac promoter, which is induced by the lactose analog isopropyl β-D-thiogalactoside (IPTG). All pGEX vectors are also engineered with an internal lacIq gene.

How is The lacIq gene used in pGEX vectors?

All pGEX vectors are also engineered with an internal lacIq gene. The lacIq gene product is a repressor protein that binds to the operator region of the tac promoter, preventing expression until induction by IPTG, thus maintaining tight control over expression of the insert.

Is the Addgene vector database a free resource?

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only – this vector is NOT available from Addgene – please contact the manufacturer for further details.